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目的探讨基质金属蛋白酶-1(MMP-1)短发夹RNA(shRNA)的慢病毒表达载体在人舌鳞癌细胞株CAL27中对MMP-1表达及细胞侵袭、转移的作用。方法设计合成用于干扰人MMP-1的shRNA并与载体PLKO.1连接,构建重组的慢病毒载体质粒PLKO.1/MMP-1-shRNA,稳定感染CAL27细胞。Western blot检测CAL27细胞中MMP-1蛋白表达,划痕实验和Transwell实验分别检测CAL27细胞的迁移和侵袭能力。结果成功构建慢病毒载体PLKO.1/MMP-1-shRNA;其能明显下调CAL27细胞中MMP-1蛋白表达,抑制CAL27细胞迁移和侵袭能力。结论慢病毒载体PLKO.1/MMP-1-shRNA能有效抑制MMP-1表达、细胞迁移和侵袭。MMP-1可能是舌鳞癌细胞转移的重要调节因子。
Objective To investigate the effect of lentivirus vector carrying MMP-1 short hairpin RNA (shRNA) on MMP-1 expression and cell invasion and metastasis in tongue squamous carcinoma cell line CAL27. Methods shRNA designed to interfere with human MMP-1 was designed and synthesized and ligated with vector PLKO.1 to construct recombinant lentiviral vector plasmid PLKO.1 / MMP-1-shRNA, which stably infected CAL27 cells. Western blot was used to detect the expression of MMP-1 in CAL27 cells. Scratch assay and Transwell assay were used to detect the migration and invasion ability of CAL27 cells. Results The lentiviral vector PLKO.1 / MMP-1-shRNA was constructed successfully. It could significantly downregulate the expression of MMP-1 in CAL27 cells and inhibit the migration and invasion of CAL27 cells. Conclusion Lentiviral vector PLKO.1 / MMP-1-shRNA can effectively inhibit the expression of MMP-1, cell migration and invasion. MMP-1 may be an important regulator of tongue squamous cell carcinoma metastasis.