分离人巨细胞病毒(HCMV)临床低传代GZ02病毒株,并根据GenBank提供的实验室标 准病毒株HCMV AD169磷酸转移酶基因UL97 DNA序列及有关文献设计引物,从HCMY GZ02病毒 株基因组DNA中通过PER扩增UL97基因,并克隆至pGEM3Z质粒载体．重组质粒经测序鉴定,发 现HCMV GZ02病毒株UL97基因序列保守区域与HCMV AD169 UL97长度完全一致,但发生G205A、 G761A、T823C、T1173C、T1282C、T1334C、T2106C等7个位点的碱基突变,涉及到编码氨基酸E69K, S275P,CA28R和F445S位点发生改变． The low passage GZ02 strain of human cytomegalovirus (HCMV) was isolated and sequenced from the genomic DNA of HCMY GZ02 strain according to the standard strain UL97 DNA of HCMV AD169 phosphatransferase gene provided by GenBank and the related primers. UL97 gene was amplified and cloned into pGEM3Z plasmid vector. The results showed that the sequence of UL97 gene of HCMV GZ02 strain was completely consistent with the length of UL97 gene of HCMV AD169, but there were 7 base mutations in G205A, G761A, T823C, T1173C, T1282C, T1334C and T2106C To coding amino acids E69K, S275P, CA28R and F445S sites changed.