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为探讨光动力疗法(PDT)对人工龋菌斑生物膜的的作用,选用变形链球菌在体外构建符合人体生理环境的人工龋模型,选择血卟啉单甲醚(HMME)为光敏剂,激光照射选用半导体激光器,波长为635 nm,输出功率为10 mW,能量密度为12.47 J/cm2。用50个釉质块形成人工龋模型,随机分成5组,每组10块,分别为:1)HMME组;2)激光照射组;3)PDT防龋组;4)0.05%(质量分数)洗必泰处理组为阳性对照;5)0.9%(质量分数)生理盐水处理组为阴性对照。依照平板细菌计数法观察不同处理方法对人工龋菌斑生物膜变形链球菌活力的影响。结果表明,与阴性对照组相比,HMME组人工龋口腔生物膜中变形链球菌数量(CFU/mL)无显著性差异(P>0.05),杀菌率仅为0.05%;激光照射和0.05%洗必泰均能使人工龋口腔生物膜中变形链球菌数量显著减少(P<0.05),杀菌率分别为59.94%和58.52%;而PDT防龋处理可使人工龋口腔生物膜中变形链球菌数量更为显著减少(P<0.05),杀菌率高达99.36%。HMME-PDT可有效杀灭人工龋口腔生物膜中的变形链球菌,为预防龋病提供了一种有效的新途径。
In order to investigate the effect of photodynamic therapy (PDT) on artificial plaque biofilm, artificial mutated Streptococcus was used to construct an artificial caries model in line with human physiological environment in vitro. Hematoporphyrin monomethyl ether (HMME) as photosensitizer, laser The semiconductor laser was irradiated at a wavelength of 635 nm with an output power of 10 mW and an energy density of 12.47 J / cm2. The artificial caries model was formed with 50 enamel blocks and randomly divided into 5 groups with 10 in each group: 1) HMME group, 2) laser irradiation group, 3) PDT caries prevention group, 4) 0.05% (mass fraction) Beitai treatment group as a positive control; 5) 0.9% (mass fraction) saline treatment group as a negative control. The effects of different treatment methods on the viability of Streptococcus mutans of human dental plaque biofilm were observed by plate bacteria counting method. The results showed that compared with the negative control group, the number of Streptococcus mutans (CFU / mL) in HMME group had no significant difference (P> 0.05), and the sterilization rate was only 0.05%. The laser irradiation and 0.05% (P <0.05), and the bactericidal rates were 59.94% and 58.52%, respectively. However, PDT could reduce the amount of Streptococcus mutans in dental caries oral biofilms More significantly reduced (P <0.05), sterilization rate as high as 99.36%. HMME-PDT can effectively kill Streptococcus mutans in dental caries oral biofilm, which provides a new effective way to prevent dental caries.