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目的观察自拟芪麦汤对糖尿病模型小鼠葡萄糖耐量的影响,体外对α-葡萄糖苷酶的抑制作用。方法四氧嘧啶高血糖模型小鼠随机分为芪麦汤低、中、高剂量组,芪麦汤低、中、高剂量联合二甲双胍组,二甲双胍组,模型对照组及正常对照组。给药30 d,结束后取血测空腹血糖,接着各组动物灌胃葡萄糖2.0 g.kg?1,测给糖后0.5,2 h血糖值,计算糖耐受量。选用芪麦汤水煎液及水煎醇提液为材料,采用体外比色法分别测定不同浓度芪麦汤药液对α-葡萄糖苷酶的抑制率。结果中、高剂量芪麦汤对糖尿病模型小鼠葡萄糖耐受量均有明显的降低作用,中、高剂量芪麦汤与二甲双胍合用均可明显降低模型小鼠葡萄糖耐受量,与单用二甲双胍比较有显著性差异。芪麦汤水煎液及水煎醇提液对α-葡萄糖苷酶均有较好的抑制作用,并有良好的量效关系,而相同剂量的水煎醇提液比水煎液的抑制率效果更好。结论自拟芪麦汤具有提高模型小鼠葡萄糖耐受量的作用。自拟芪麦汤对α-葡萄糖苷酶的抑制作用,提示了该方降糖作用的可能机制之一。
Objective To observe the effects of self-made Qimai Tang on impaired glucose tolerance in diabetic mice and the inhibitory effect on α-glucosidase in vitro. Methods Alloxan-induced hyperglycemia mice were randomly divided into low, medium and high dose of Qimai Tang group, low, medium and high dose of Qimai Tang combined with metformin group, metformin group, model control group and normal control group. Administration 30 d, after the end of fasting blood glucose measurement, followed by glucose in rats fed each group 2.0 g.kg? 1, measured 0.5, 2 h after the blood glucose, glucose tolerance calculated. The Qimai Decoction and the decoction of alcohol extract were used as materials. The inhibitory rates of α-glucosidase were determined by colorimetric method in vitro. Results, high-dose Qimai Tang diabetic mice glucose tolerance were significantly reduced, medium and high doses of Qimai Tang combined with metformin significantly reduce glucose tolerance in model mice, and metformin alone More significant differences. Decoction of Qimai decoction and decoction of alcohol extract had better inhibitory effect on α-glucosidase, and had a good dose-effect relationship, but the same dosage of decoction of decoction alcohol decoction than the decay rate effect better. Conclusion Self-made Qimai Tang has the effect of increasing glucose tolerance in model mice. Self-prepared Qimai decoction of α-glucosidase inhibition, suggesting that the side effects of hypoglycemic one of the possible mechanisms.