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采用ELISA方法对25例慢性肾功衰竭(Chronicrenalfailure,CRF)非透析治疗患者,6例健康对照者外周血单个核细胞(Peripheralbloodmononuclearcel,PBMC)产生肿瘤坏死因子(Tumornecrosisfactor,TNF)的水平进行了观察。结果:CRF患者PBMC在有无细菌脂多糖(Lipidpolysaccharide,LPS)刺激下均较正常对照组产生TNF明显升高;氮质血症期、尿毒症期的CRF患者PBMC分泌TNF的水平无显著性差异。揭示CRF患者PBMC处于激活状态并分泌TNF。
Tumor necrosis factor (TNF) levels in peripheral blood mononuclear cells (PBMCs) from 25 patients with chronic renal failure (CRF) without dialysis and 6 healthy controls were measured by ELISA. Results: Compared with normal control group, the PBMC of CRF patients had significantly higher TNF production than that of normal control group in the presence or absence of bacterial lipopolysaccharide (LPS) stimulation; there was no significant difference of TNF secretion in CRF patients during azotemia and uremia . It is revealed that PBMCs in CRF are activated and TNF secreted.