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酶联免疫吸附试验(ELISA)具有特异性好、高效稳定、适合医院大批量标本检测等优点。但ELISA试验影响因素较多、步骤偏多,稍有疏忽就会影响试验结果[1]。所以,必须加强质量控制,做到标准操作,才能确保检测结果准确可信。综合国内外资料,并结合作者的工作经验,谈几点体会。1试验环境ELISA试剂中,碱性磷酸酶或者辣根过氧化物酶都是光敏感性酶,光密度单位会随着温度的改变而变化。因而室温对检
Enzyme-linked immunosorbent assay (ELISA) with good specificity, high efficiency and stability, suitable for large-volume hospital specimens detection and so on. However, there are many influencing factors in ELISA test, more steps and a little negligence will affect the test results [1]. Therefore, we must strengthen quality control, standard operation, to ensure that the test results are accurate and credible. Comprehensive domestic and foreign information, combined with the author’s work experience, talk a few experiences. A test environment ELISA reagents, alkaline phosphatase or horseradish peroxidase are light-sensitive enzymes, optical density units will change with temperature changes. Thus room temperature on the seizure