我们在使用小柱层析法分离血清天冬氨酸氨基转移酶线粒体同功酶(m-AST)的基础上,结合以前分离肌酸激酶同功酶MB(CK-MB)的方法,作了若干改进,在一根小柱上同时分离m-AST及CK-MB。现将方法介绍如下: We used the column chromatography to separate the serum aspartate aminotransferase mitochondrial isoenzyme (m-AST) and combined it with the previous method of separating the creatine kinase isoenzyme MB (CK-MB). Several improvements were made to separate m-AST and CK-MB simultaneously on a small column. The method is now described as follows: