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本文根据扫描电镜观察,报告了dbcAMP引起小鼠U_(14)腹水瘤细胞微绒毛和质膜表面的细微形态变化。U_(14)腹水瘤的多数细胞体积大,大小相近,表面有许多细长微绒毛;微绒毛表面和微绒毛间细胞质膜表面,仅见很细微的点状微突。腹腔内注射dbcAMP的U_(14)腹水瘤,85％以上的细胞体积缩小,大小不等,表面微绒毛缩短且减少。在没有微绒毛的部位,细胞质膜表面有许多颗粒状微突起,较对照组明显易见。微绒毛表面也有与之大小类似的微突起,使微绒毛表面凹凸不平。流式细胞光度计分析结果表明,在本文实验条件下,dbcAMP未引起U_(14)腹水瘤细胞周期的变化,细胞膜表面和微绒毛的变化,也不单纯是细胞周期中微绒毛数量和分布的改变。因此作者认为,这些变化不是通过改变细胞周期引起的。 cAMP引起微绒毛退缩的机制不详。至于微绒毛间的颗粒状微突起是微绒毛退缩的遗迹,抑或是由cAMP引起,尚待研究。cAMP引起瘤细胞表面形态的变化,进一步证明了cAMP在小鼠体内诱导恶性细胞分化的作用。
In this paper, based on the scanning electron microscopy, dbcAMP induced mouse U_ (14) ascites tumor cells microvilli and plasma membrane surface morphology changes. Most of U_ (14) ascites tumor cells were large in size and similar in size, with many elongated microvilli on the surface. On the surface of the plasma membrane of the microvilli and microvilli, only tiny subtle micropolar processes were observed. Intraperitoneal injection of dbcAMP U_ (14) ascites tumors, more than 85% of the cells shrink in size, size, surface microvilli shortened and reduced. In the absence of microvilli on the surface of the plasma membrane there are many granular microprojections, compared with the control group is obvious. Microvilli surface also has a similar size of microprojections, microvilli surface uneven. Flow cytometry analysis showed that under the experimental conditions, dbcAMP did not change the cell cycle of U 14 ascites tumor cells and the changes of cell surface and microvilli were not simply the number and distribution of microvilli in the cell cycle change. Therefore, the author believes that these changes are not caused by changing the cell cycle. The mechanism by which cAMP induces microvilli retraction is unknown. Whether micro-prominence between microvilli remains a relict of microvilli or is caused by cAMP remains to be studied. cAMP caused changes in the surface morphology of tumor cells, further demonstrating the role of cAMP in inducing malignant cell differentiation in mice.