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由水稻组织培养后代选育的品种黑珍米与亲本Basmati 370相比,其形态性状,如株高,每穗总粒数,穗长,单株有效穗数,千粒重和生育期等发生了显著变化;并且使原来的白稃尖和白谷壳变成了紫稃尖和紫色谷壳,应用分布于12条水稻染色体的121个DNA探针分析黑珍米与其亲本的RFLP,结果表明:有24.8％的探针检测到了多态性,分布于10条染色体。结合4种限制性内切酶(EcoRⅠ,BamHⅠ,HindⅢ和XbaⅠ)分析的67个DNA探针中,18个探针检测到多态性,其中14个只能在一种酶检测到多态性,是由点突变引起;而其他4个能在3种或4种酶同时检测到多态性,是由插入或缺失引起。说明组培产生的无性系变异主要来源于点突变。
The morphological traits such as plant height, total grain number per panicle, panicle length, effective panicle per plant, 1000-grain weight and growth period were significantly different from those of Basmati 370 The original white tips and white chaff transformed into purple tip and purple chaff. The 121 DNA probes distributed in 12 rice chromosomes were used to analyze the RFLP of Heianzao and its parents. The results showed that there were 24.8 % Of the probes detected polymorphisms and distributed on 10 chromosomes. Of the 67 DNA probes analyzed by 4 restriction enzymes (EcoRⅠ, BamHⅠ, HindⅢ and XbaⅠ), 18 probes detected polymorphisms, 14 of which could only detect polymorphisms in one enzyme , Is caused by point mutations; while the other four can be detected in 3 or 4 kinds of enzyme polymorphism, is caused by the insertion or deletion. The results showed that clonal variation in tissue culture mainly originated from point mutation.