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自Ohytil等首先提出各种组织中的染色质非组蛋白有免疫特异性后,曾就种属、生长期等进行了各方面的研究Yeoman等证明在Novikoff肝癌和Walker256肉瘤的染色质中具有免疫性相同的癌组织特异的非组蛋白。上述工作大多采用补体结合试验及双相免疫扩散作为鉴定法,而这两种方法的灵敏度不高,受到影响的因素较多,不易得到明确的结果。本文选择敏感度较高的免疫荧光法及免疫辣根过氧化物酶标(简称免
Since Ohytil et al. First proposed the immunospecificity of chromatin non-histone proteins in various tissues, various studies have been carried out on the species and the growth period. Yeoman et al. Demonstrated immunity in the chromatin of Novikoff hepatocarcinoma and Walker256 sarcoma The same cancerous tissue-specific non-histone. Most of the above work using complement fixation test and two-phase immunodiffusion as the identification method, and the sensitivity of these two methods is not high, affected by many factors, not easy to get a clear result. This selection of high sensitivity immunofluorescence and immune horseradish peroxidase standard (referred to as free