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目的探讨人参皂苷Rh2(ginsenoside Rh2,G-Rh2)能否提高人乳腺癌细胞MCF-7对5-氟尿嘧啶(5-fluorouracil,5-FU)的敏感性,通过协同作用扩大细胞凋亡信号。方法先用MTT法确定G-Rh2的无毒增敏质量浓度,随后将对数生长的人乳腺癌细胞MCF-7分为4组:control(不加药),G-Rh2(加入10 mg.L-1 G-Rh2),5-FU(加入25 mg.L-1 5-FU),G-Rh2+5-FU组(加入10 mg.L-1 G-Rh2和25 mg.L-15-FU),通过MTT计算联合作用指数,激光共聚焦观察细胞形态学变化,流式细胞术检测细胞周期,免疫荧光染色检测caspase-3、多聚ADP核糖聚合酶(poly ADP-ribose polymer-ase,PARP)的剪切情况。结果 MTT结果显示,G-Rh2可导致5-FU诱导的人乳腺癌细胞MCF-7死亡率增加,形态学、细胞周期检测、caspase-3和PARP凋亡蛋白免疫荧光染色结果都显示,G-Rh2+5-FU组可显著提高人乳腺癌细胞MCF-7死亡率,且引起的细胞死亡为细胞凋亡作用。结论 G-Rh2可以提高人乳腺癌细胞MCF-7对5-FU的敏感性,通过协同作用诱导细胞凋亡。
Objective To investigate whether ginsenoside Rh2 (G-Rh2) can enhance the sensitivity of human breast cancer cell line MCF-7 to 5-fluorouracil (5-FU) and to increase the signal of apoptosis through synergistic effect. Methods The non-toxic sensitized mass concentration of G-Rh2 was determined by MTT method. Then the logarithmic growth of human breast cancer cells MCF-7 were divided into 4 groups: control (without drug), G-Rh2 (10 mg. L-1 G-Rh2), 5-FU (25 mg.L-1 5-FU), G-Rh2 + 5-FU group (10 mg.L-1 G-Rh2 and 25 mg.L-15 -FU). MTT was used to calculate the combined effect index. The morphological changes of the cells were observed by laser scanning confocal microscope. The cell cycle was detected by flow cytometry. The expressions of caspase-3, poly ADP-ribose polymer-ase , PARP) shear. Results The results of MTT showed that G-Rh2 could increase the death rate of MCF-7 cells induced by 5-FU, and the results of immunofluorescence staining of morphological, cell cycle, caspase-3 and PARP proteins showed that G- Rh2 + 5-FU group can significantly increase the human breast cancer cell MCF-7 mortality, and cell death caused by apoptosis. Conclusion G-Rh2 can enhance the sensitivity of human breast cancer cell line MCF-7 to 5-FU and induce apoptosis through synergistic effect.