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目的 :探讨一氧化氮 (NO)在L 精氨酸 (L arg)所致肾大部切除 (SNx)大鼠残余肾代偿性增生中的作用。方法 :采用 5 / 6肾大部切除大鼠为实验模型 ,实验组于手术后分别给予 1%L arg或NO供体Molsidomine (MSD)。实验分为假手术组 (Sham)、SNx组、SNx加L arg组和SNx加MSD组 ,观察指标为体重 (BW )、残余肾重 (KW)、肾重 /体重 (KW /BW )、尿蛋白定量、血压、Ccr、残余肾代偿性增生比率 (CRG)、残余肾蛋白质、DNA含量、小管间质细胞PCNA免疫组化表达、尿NO代谢产物NO2 -排泄量等。结果 :L arg组大鼠残余肾代偿性增生指标 (KW、KW /BW、CRG、蛋白质、DNA及PCNA等 )较其对照组明显增加 (P小于 0 .0 5或 0 .0 0 1) ,MSD组上述指标与SNx组相比无显著统计学差异 (P >0 .0 5 )。L arg及MSD组NO2 -排泄量均较SNx组显著增加。结论 :L arg可刺激大鼠残余肾代偿性增生 ,这种作用可能与NO无关
Objective: To investigate the role of nitric oxide (NO) in the remnant renal compensatory proliferation induced by L-arginine (L arg) in subtotal nephrectomized rats. Methods: A total of 5/6 nephrectomized rats were used as the experimental model. The experimental group received 1% L arg or NO donor Molsidomine (MSD) respectively after operation. The experiment was divided into Sham group, SNx group, SNx plus L arg group and SNx plus MSD group. The indexes of observation included BW, KW, KW / BW, Protein, blood pressure, Ccr, residual renal compensatory proliferative ratio (CRG), residual renal protein, DNA content, PCNA expression of tubulointerstitial cells and NO2 - excretion of urinary NO metabolites. Results: The indexes of residual renal compensatory proliferation (KW, KW / BW, CRG, protein, DNA and PCNA) in L arg group were significantly higher than those in control group (P less than 0.05 or 0.0100) There was no significant difference between the above indexes in MSD group and SNx group (P> 0.05). NO2 - excretion in L arg group and MSD group was significantly higher than that in SNx group. Conclusion: L arg can stimulate compensatory proliferation of residual kidney in rats, which may not be related to NO