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目的:探讨氯通道在5-氟尿嘧啶(5-Fu)诱导的低分化鼻咽癌细胞CNE-2Z凋亡性细胞容积减小(AVD)和细胞凋亡中的作用。方法:培养CNE-2Z细胞后,分别用100μmol/L5-Fu(5-Fu组)、100μmol/L5-Fu+100μmol/L5-硝基-2-(3-苯丙胺)苯甲酸(5-Fu+NPPB组)处理细胞,采用活细胞影像系统实时拍摄细胞图像,检测细胞容积变化,Hochest33258荧光染色技术检测细胞凋亡并计算细胞凋亡率。结果:5-Fu处理使细胞皱缩,体积变小;5-Fu+NPPB处理后细胞体积变化不明显。在5个不同时间点,细胞受到5-Fu刺激后,标准化细胞容积(Vst)均小于对照组,5-Fu+NPPB组对细胞Vst的影响均小于5-Fu组,差异均有统计学意义。对照组细胞凋亡率(1.8±0.5)%,5-Fu处理使细胞凋亡率增加至(49.2±2.6)%,5-Fu+NPPB处理使细胞凋亡率降至(12.5±2.9)%。结论:抑制氯通道可显著拮抗5-Fu诱导的凋亡性细胞容积减小和细胞凋亡。
Objective: To investigate the role of chloride channel in 5-Fu induced CNE-2Z apoptotic cell volume decrease (AVD) and apoptosis in poorly differentiated nasopharyngeal carcinoma cells. Methods: After cultured CNE-2Z cells, the cells were treated with 100μmol / L 5-Fu (5-Fu group), 100μmol / L 5-Fu + 100μmol / L 5-Fu + NPPB group) were used to treat the cells. The live cell imaging system was used to take real-time images of the cells and detect the changes of the cell volume. Hochest33258 fluorescence staining was used to detect the apoptosis and calculate the apoptosis rate. Results: After 5-Fu treatment, the cell shrinkage and volume became smaller. The volume of 5-Fu + NPPB treatment did not change obviously. After 5-Fu stimulation, the normalized cell volume (Vst) was less than that of the control group at 5 different time points. The effect of 5-Fu + NPPB group on Vst was lower than that of 5-Fu group, the difference was statistically significant . In the control group, the apoptotic rate was (1.8 ± 0.5)%, the apoptosis rate was increased to (49.2 ± 2.6)% after 5-Fu treatment, and the apoptotic rate was reduced to (12.5 ± 2.9)% . Conclusion: Inhibition of chloride channels can significantly antagonize 5-Fu-induced apoptotic cell apoptosis and apoptosis.