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将木质纤维素原料转化为燃料乙醇,受到全世界的期待。本研究探讨了利用复合菌系直接分解糖化的新途径。结果显示,该复合菌系优化的产酶活条件是转速为0r/min,底物添加量为3%,第6天酶活达到峰值,主要酶活为木聚糖酶活、CMC酶活和滤纸酶活,分别为15.18、3.43和0.79IU/mL。在第15天培养结束后,WSD-5对小麦秸秆中纤维素、半纤维素以及木质素的分解率分别达到71.3%、90.6%和51.7%。在酶活高峰期,在添加底物的同时升温至55℃保温糖化,结果发现还原糖的浓度显著提高。酶解小麦秸杆产生的总还原糖达到125mg/g以上,两次糖化总还原糖浓度为常温发酵时的15倍以上。当底物浓度为6%时的转化率最高,达到150.73mg/g。在糖化的过程中,产生了少量的甲酸、乙酸等可溶性小分子化合物,可以作为甲烷发酵的底物,具有一定的利用意义。试验扩大到3m3规模的中试试验。本研究为木质纤维素的低成本分解糖化提供了新的思路和途径。
The conversion of lignocellulosic feedstock to fuel ethanol has been the world’s expectation. This study explored the use of complex strains of direct decomposition of saccharification of new ways. The results showed that the optimum conditions for enzyme production were 0r / min rotation speed, 3% substrate addition, and peak activity on the 6th day. The main enzyme activities were xylanase activity, CMCase activity and Filter paper enzyme activity, respectively 15.18,3.43 and 0.79IU / mL. At the end of the 15th day, the decomposition rate of WSD-5 in wheat straw reached 71.3%, 90.6% and 51.7% respectively. During the peak of enzyme activity, the temperature was raised to 55 ° C while adding the substrate, and the temperature was increased. The concentration of reducing sugar was significantly increased. The total reducing sugars produced by enzymatic hydrolysis of wheat straw reached above 125mg / g, and the total twice saccharified reducing sugar concentration was 15 times higher than that at room temperature. The conversion reached the highest at 6% substrate concentration, reaching 150.73 mg / g. In the process of saccharification, a small amount of soluble small molecule compounds such as formic acid and acetic acid are produced, which can be used as substrate for methane fermentation and have certain utilization significance. Test extended to 3m3 scale pilot test. This study provides a new way for the low-cost decomposition and saccharification of lignocellulose.