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用硝酸钙添加剂形成难熔的氟化钙，高温灰化进行样品前处理。以柠檬酸钠—氯化物—冰醋酸所组成的总离子强度调节剂（ＴＩＳＡＢ）的ｐＨ值控制在５．０～５．５之间，用氟离子选择电极测定生物样品中氟含量，间接求出５－ＦＵ、ＦＴ－２０７。可测血浆氟线性范围０．００５～０．０５和０．０５～２０μｇ／ｍｌ。相关系数为０．９９９８和０．９９９１，检测下限０．００５μｇ／ｍｌ．血浆５－ＦＵ可测范围０．００～６８４０．３９μｇ／ｍｌ。测定血浆中氟及５－ＦＵ标准差为０．００５、０．０８；变异系数为２．３％、４．５％；回收率为９７．５％、８４．０～１１１．２％。对大剂量口服ＦＴ－２０７的狗，测定内脏ＦＴ－２０７，获满意结果。本法也适用于其它含氟药物的测定
A refractory calcium fluoride is formed with calcium nitrate additive and the sample is pre-treated at high temperature ashing. The total ionic strength regulator (TISAB) composed of sodium citrate-chloride-glacial acetic acid is controlled at a pH between 5.0 and 5.5. Fluorine ion selective electrode is used to determine the content of fluorine in biological samples. Out of 5-FU, FT-207. Measurable linear range of plasma fluorine 0.005 ~ 0.05 and 0.05 ~ 20μg / ml. The correlation coefficient was 0.9998 and 0.9991, the detection limit was 0.005μg / ml. Plasma 5-FU can measure the range of 0.00 ~ 6840.39μg / ml. The standard deviation of plasma fluoride and 5-FU was 0.005, 0.08; the coefficient of variation was 2.3% and 4.5%; the recoveries were 97.5% and 84.0 ~ 111.2% respectively. For high-dose oral FT-207 dogs, visceral FT-207 was measured and satisfactory results were obtained. This Law is also applicable to the determination of other fluorine-containing drugs