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目的 :观察壳聚糖加中药首乌、丹参和牛膝对实验性脂肪肝大鼠的预防作用。方法 :在小剂量四氯化碳致肝损伤的基础上 ,合并应用高脂饮食复制大鼠脂肪肝模型 ,同时分别给予不同浓度的药物 ,以肝脏的脂质含量为衡量标准 ,并应用计算机彩色图象分析技术测量脂肪变性 (脂变 )面积 ,观察药物预防效果。结果 :四氯化碳损伤肝脏合并高脂饮食可引起大鼠肝脏明显的脂变 ,表现为肝组织三酰甘油 (TG)、总胆固醇 (TC)含量显著增加 ,SOD活性明显降低 ,病理切片检查显示肝细胞肿胀 ,内部充满大小不等的脂滴 ,而药物防治组大鼠与模型组相比肝组织TG和TC值显著降低 ,肝细胞的脂变程度明显小于模型组 ,表现为脂滴减少 ,脂变面积缩小 ,SOD活性逐渐升高并恢复正常 ,其改善程度随用药剂量的增加而增加。结论 :该药物对脂肪肝的形成具有明显的预防作用。
Objective: To observe the preventive effect of Chitosan plus Radix Polygonum multiflorum, Radix Salviae Miltiorrhizae and Achyranthes bidentata on experimental fatty liver in rats. Methods: On the basis of low dose carbon tetrachloride-induced liver injury, a rat model of fatty liver was established by high-fat diet combined with different concentrations of drugs. The lipid content of the liver was taken as the standard, and computer color Image analysis techniques to measure the area of steatosis (lipid change), observe the effect of drug prevention. Results: CCl 4 -induced liver combined with high-fat diet can cause significant hepatic steatosis in rats. The liver tissue showed triglyceride (TG), total cholesterol (TC) content increased significantly, SOD activity decreased significantly, pathological examination Showing liver cell swelling, internal filled with lipid droplets of varying sizes, while the drug control group compared with the model group, liver TG and TC values were significantly lower, the degree of hepatocellular lipidosis was significantly less than the model group, showed lipid droplets decreased , The area of lipid change narrowed, the activity of SOD gradually increased and returned to normal, the degree of improvement increased with the increase of dosage. Conclusion: The drug has a significant preventive effect on the formation of fatty liver.