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Objective This study aimed to investigate the expression and significance of PIN2/TERF1 interacting, telomerase inhibitor 1(Pin X1) and human telomerase reverse transcriptase(h TERT) in basal cell carcinoma(BCC). Methods Real-time polymerase chain reaction and immunohistochemistry were performed to quantify the m RNA expressions and integrated optical density(IOD), respectively, of Pin X1 and h TERT in BCC specimens(n = 30), as well as in normal skin specimens(n = 15). Results The m RNA expression level and IOD of Pin X1 in the BCC samples were both significantly lower than those in the control specimens(P < 0.05). Conversely, the m RNA expression level and IOD of h TERT in BCC were both significantly higher than that in the control samples(P < 0.05). The correlation between the expression levels of Pin X1 and h TERT showed no statistical significance(P > 0.05). Conclusion Downregulation of Pin X1 and upregulation of h TERT expression may be associated with the activation and maintenance of telomerases in the induction of BCC.
Objective This study aimed to investigate the expression and significance of PIN2 / TERF1 interacting, telomerase inhibitor 1 (Pin X1) and human telomerase reverse transcriptase (h TERT) in basal cell carcinoma (BCC). Methods Real-time polymerase chain reaction and immunohistochemistry were Perform to quantify the m RNA expressions and integrated optical density (IOD), respectively, of Pin X1 and h TERT in BCC specimens (n = 30), as well as in normal skin specimens (n = 15). Results The m RNA expression level and IOD of Pin X1 in the BCC samples were both significantly lower than those in the control specimens (P <0.05). Conversely, the m RNA expression level and IOD of h TERT in BCC were both significantly higher than that in the control samples (P <0.05). The correlation between the expression levels of Pin X1 and h TERT showed no statistical significance (P> 0.05). Conclusion Downregulation of Pin X1 and upregulation of h TERT expression may be associated with the activation and maintenan ce of telomerases in the induction of BCC.