随着 PCR和 RAPD技术在生物学领域的广泛应用 ,人们在实验中遇到的问题也越来越多 ,其中RAPD的可重复性差是最主要的一个问题 ,也是该技术最难克服的缺点。作者在利用 RAPD技术标记进行小麦的抗病毒基因研究中 ,对影响扩增结果的模板、底物、引物和扩增程序等进行了实验探索 ,结果表明 :模板的浓度、d NTP浓度、引物浓度及反应程序均对 RAPD扩增结果有明显影响。 With the extensive application of PCR and RAPD in the field of biology, there are more and more problems encountered in the experiment. The poor repeatability of RAPD is the most important issue and the most difficult to overcome. In the study of antiviral genes of wheat by RAPD technology, the experiments on templates, substrates, primers and amplification programs affecting the amplification results were carried out. The results showed that the template concentration, dNTP concentration, primer concentration And the reaction procedure have a significant impact on the results of RAPD amplification.