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基于在0.02 mol/L硫酸中,硒(IV)与过量的I-反应生成I3-,I3-进一步与吖啶橙(AO)反应生成1∶1离子缔合物,体系吸光度明显增强,建立了分光光度法测定痕量硒(IV)的新方法。试验了酸度、试剂用量、表面活性剂、温度和时间的影响,确定了最佳测定条件。最大吸收峰位于491 nm。线性范围为0μg/L~56μg/L。检出限为1.07μg/L。大量IO3-干扰可用盐酸羟胺消除。方法用于硒强化营养盐和健康平衡盐中硒含量的测定,结果与荧光法测定值一致,相对标准偏差为0.61%~0.70%(n=5),加标回收率为100.4%~100.8%。
Based on the reaction of selenium (IV) with excess I- in 0.02 mol / L sulfuric acid to form I3-, I3- further reacted with acridine orange (AO) to form a 1: 1 ion association complex. A New Spectrophotometric Method for the Determination of Trace Selenium (IV). The effects of acidity, reagent dosage, surfactant, temperature and time were tested to determine the best assay conditions. The maximum absorption peak is at 491 nm. The linear range was 0μg / L ~ 56μg / L. The detection limit was 1.07μg / L. A large number of IO3-interference can be eliminated with hydroxylamine hydrochloride. The method was applied to the determination of selenium in selenium fortified nutrient and healthy balanced salt. The results were consistent with those obtained by fluorescence spectrometry. The relative standard deviations were 0.61% -0.70% (n = 5), and the recoveries were 100.4% -100.8% .