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Objective:To report high co-positivity of anti-dengue virus(DV)and anti-Japanese encephalitis virus(JEV)IgM in an area endemic for both the viruses and to discuss the possibilities of coinfection.Methods:Serum samples from the patients who presented with fever,suspected central nervous system infection and thrombocytopenia,were tested for anti-DV IgM and antiJEV IgM antibodies.Conventional reverse transcriptase polymerase chain reaction was done for detection of DV RNA and JEV RNA.Results:Of 1 410 patient sera tested for anti-DV and antiJEV antibodies,129(9.14%)were co-positive for both.This co-positivity was observed only in those months when anli-JEV IgM positivily was high.Tilers of both anli-DV IgM and anti-JEV IgM were high in most of the co-positive eases.Among these 129 co-positive cases,76 were lesled by conventional reverse Iranscriplase polymerase chain reaction for both flaviviruses,of which eight cases were co-positive for DV and JEV.Conclusions:Co-infection with more than one fluvivirus species can occur in hyperendemic areas.
Objective: To report high co-positivity of anti-dengue virus (DV) and anti-Japanese encephalitis virus (JEV) IgM in an area endemic for both the viruses and to discuss the possibilities of coinfection. Methods: Serum samples from the patients who presented with fever, Suspected central nervous system infection and thrombocytopenia, were tested for anti-DV IgM and antiJEV IgM antibodies. Conventional reverse transcriptase polymerase chain reaction was done for detection of DV RNA and JEV RNA. Results: Of 1 410 patient sera tested for anti-DV and antiJEV antibodies, 129 (9.14%) were co-positive for both.This co-positive was observed was only in those months when anli-JEV IgM positivily was high. Tilers of both anli-DV IgM and anti-JEV IgM were high in most of the co-positive eases. Among these 129 co-positive cases, 76 were lessed by conventional reverse Iranscriplase polymerase chain reaction for both flaviviruses, of which eight cases were co-positive for DV and JEV. Conclusions: Co- infection with more than one fluvivirus species can occur in hyperendemic areas.