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将硅胶表面硅羟基与γ-(甲基丙烯酰氧)丙基三甲氧基硅烷反应合成具有CC端基的改性硅胶核,进一步以N-Boc-L-色氨酸为模板分子,采用分子印迹技术在改性后的硅胶表面包覆印迹聚合物,制备出核壳结构氨基酸分子印迹分离介质。这种印迹分离介质对模板N-Boc-L-色氨酸具有良好的吸附性,最大饱和吸附量可达到85.96 mg/g,印迹效率为70.67%,明显高于本体聚合法。采用新型分子印迹分离介质装填成高效液相色谱柱,实现了模板分子与其对映体的快速基线分离,分离度为1.64。
The modified silica gel core with CC terminal was synthesized by reacting silanol groups with γ- (methacryloyloxy) propyltrimethoxysilane on the surface of silica gel, and further using N-Boc-L-tryptophan as template molecule, The imprinted polymer was coated on the surface of the modified silica gel by molecular imprinting technology to prepare a core-shell molecular imprinted polymer. This imprinted separation medium has good adsorption on the template N-Boc-L-tryptophan, with the maximum adsorption capacity of 85.96 mg / g and the imprinting efficiency of 70.67%, which is obviously higher than that of bulk polymerization. A new molecular imprinting separation medium was loaded into high performance liquid chromatography (HPLC) column to achieve rapid baseline separation of the template molecule and its enantiomer. The resolution was 1.64.