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本文比较了柑桔属、金柑属、枳属的57个种和栽培品种的蛋白质组分Ⅰ的大亚基(LS)和小亚基(SS),以分析它们之间的系统发育关系。供试样品全展叶的蛋白质组分Ⅰ电泳结果表明,LS有两种类型,各由三条多肽带组成;LSⅠ的等电点较LSⅡ更偏于碱性。除枸橼呈LSⅡ,其余供试的种和栽培品种概呈LSⅠ。蛋白质组分Ⅰ的SS由标记为a、b、c、d、e的五条多肽带组成,惟SSe的等电点比LS偏酸。 根据大、小亚基的结合情况,柑桔属及其近缘属的蛋白质组分Ⅰ的等电聚焦图谱可分成六组。第一组由LSⅠ和SSc和d组成,包括柑桔属的绝大多数种和栽培品种。第二组由LSⅠ和SSd组成,包括多数的宽皮柑桔品种。第三组由LSⅠ和SSc组成,包括金柑属种类,马蜂柑、卡西大翼橙、柠檬和柚。第四组由LSⅠ和SSb及与b相当接近
In this paper, we compared the large subunit (LS) and small subunit (SS) of 57 species and cultivars of citrus, kumquat and citrus to analyze their phylogenetic relationships. The results of electrophoresis of protein fraction I in the whole leaf showed that there are two types of LS, each consisting of three polypeptide bands; the isoelectric point of LSⅠis more alkaline than that of LSⅡ. In addition to citron was LS Ⅱ, the remaining test species and cultivars were LS Ⅰ. The SS of protein fraction I consists of five polypeptide bands labeled as a, b, c, d, e, except that the SSe has an isoelectric point that is slightly more acidic than LS. According to the combination of large and small subunits, the isoelectric focusing spectra of the protein component I of Citrus and its relatives can be divided into six groups. The first group consists of LSI and SSc and d, including most species and cultivars of the genus Citrus. The second group consists of LSI and SSd, including the majority of broad-leaved citrus varieties. The third group consists of LSI and SSc, including kumquat genus, mandarin, cassia aurora, lemon and grapefruit. The fourth group consists of LSI and SSb and is fairly close to b