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目的 抑癌基因的杂合缺失被认为是结直肠癌形成的关键步骤之一 ,本研究利用微卫星DNA标记杂合缺失分析法对散发性结直肠癌 2 0号染色体杂合缺失情况进行研究 ,并探讨其意义。方法 83例结直肠癌病人的肿瘤及正常组织的DNA ,经PCR反应后 ,以ABIPrism 3 77自动荧光测序仪进行电泳 ,以GeneScan2 1和Genotyper3 1软件进行基因分型。结果 整条染色体的平均杂合缺失率为 2 2 8% ,2 0号染色体短臂平均杂合缺失率为 2 6 7% ,长臂平均杂合缺失率为 2 1 1 %。 2 0号染色体整个短臂及长臂 2 0q1 1 1至 2 0q1 3 1均表现出很高的杂合缺失率。结论 在散发性结直肠癌中 2 0号染色体存在明显的基因组不稳定现象 ,提示结直肠癌相关基因的存在
Objective The heterozygosity deletion of tumor suppressor gene is considered as one of the key steps in the formation of colorectal cancer. In this study, heterozygous deletion of sporadic colorectal cancer chromosome 20 was studied by microsatellite DNA marker heterozygous deletion analysis, And explore its meaning. Methods 83 cases of colorectal cancer patients with tumor and normal tissue DNA, PCR reaction, the ABIPrism 3 77 automatic fluorescence sequencer electrophoresis, GeneScan2 1 and Genotyper3 1 software for genotyping. Results The average rate of heterozygosity deletion in the entire chromosome was 22.8%. The average heterozygous deletion rate of the short arm on chromosome 20 was 26.7% and the average length of the long arm was 21.1%. Chromosome 20 has a high rate of heterozygosity for the entire short arm and long arm 20q11-1 to 20ql31. Conclusions In chromosome 20 of sporadic colorectal cancer, there is obvious genomic instability, suggesting the existence of colorectal cancer related genes