一、引言纸上电泳是一种物理化学的研究方法,很早就用于测量离子的电荷种类和迁移率。但是A.Tiselius指出,用这种方法不能得到准确的迁移率值,因为在实验中由于蒸发、电渗及电场强度的变化,有时会导致假的结果。H.Kunkel指出,在封闭式纸上电泳中,如果校正了电渗,就可以得到满意的结果。R.Audubert提出在开放式的纸条上对缓冲液的流动进行校正,并利用积分法计算离子的迁移率。我们在采用封闭式纸上电泳测定离子迁移率时,发现缓冲液在纸条上的虹吸效应对离 I. INTRODUCTION Paper electrophoresis is a physico-chemical research method that has long been used to measure ion species and mobilities. However, A. Biselius pointed out that accurate mobility values ​​can not be obtained with this method because in experiments the false results can sometimes be caused by changes in evaporation, electroosmosis and electric field strength. H.Kunkel pointed out that in the closed paper electrophoresis, if the correction of electroosmosis, you can get satisfactory results. R. Audubert proposed to correct the flow of buffer on an open strip of paper and calculate the ion mobility using the integral method. We used the closed-cell paper electrophoresis determination of ion mobility, the buffer was found in the siphon effect on the paper