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目的探讨食管鳞状上皮细胞癌（食管鳞癌）中细胞凋亡的表达及其临床意义。方法应用末端脱氧核苷酸转移酶介导的ｄＵＴＰ缺口末端标记（ＴＵＮＥＬ）法，研究人食管鳞癌细胞凋亡的表达水平。结果３８例手术切除的食管鳞癌组织平均细胞凋亡指数ＡｐｏＬＩ为１２．１０±５．１３‰。角化型ＡｐｏＬＩ高于非角化型（Ｐ＜０．０１）；肿瘤分化越好，细胞凋亡越多，低度、中度、高度分化ＡｐｏＬＩ有差异（Ｐ＜０．０５）；不同性别、年龄、肿瘤部位、长度、深度，其ＡｐｏＬＩ并无差异；以１０‰为界将ＡｐｏＬＩ分组绘制Ｋａｐｌａｎ－Ｍｅｉｅｒ生存曲线，ＡｐｏＬＩ≥１０‰组术后生存率高。结论细胞凋亡与原发食管鳞癌临床病理特征密切相关，并可用于食管鳞癌预后判断。
Objective To investigate the expression of apoptosis in esophageal squamous cell carcinoma (esophageal squamous cell carcinoma) and its clinical significance. Methods Endodeoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to study the expression level of apoptosis in human esophageal squamous cell carcinoma cells. Results The average apoptotic index (ApoLI) of esophageal squamous cell carcinoma in 38 patients was 12.10±5.13 ‰. The keratinized ApoLI was higher than non-keratinized (P<0.01); the better the tumor differentiation, the more apoptosis, the lower, moderate, and highly differentiated ApoLI (P<0.05); different sex There was no difference in ApoLI between age, tumor site, length, and depth. ApoLI was grouped into circles to draw Kaplan-Meier survival curves with 10 ‰. The ApoLI ≥ 10 ‰ group had a high survival rate. Conclusions Apoptosis is closely related to the clinicopathological features of primary esophageal squamous cell carcinoma and can be used for the prognosis of esophageal squamous cell carcinoma