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从杭州市郊的大豆病株上获得一病毒分离物Ｓ１，温室人工摩擦接种７科１６种植物，Ｓ１能侵染其中的５科１４种植物．Ｓ１能由桃蚜、豆蚜以非持久性方式传毒，该分离物的钝化温度为５０～５５℃，稀释限点为１０－３～１０－４，体外保毒期为３～４ｄ（２５℃）．用昆诺藜作繁殖寄主可提到大量球状病毒粒子，ｄ为２５～３０ｎｍ．病毒外壳蛋白主要由两种亚基构成，Ｍｒ分别为４４．７×１０３和２１．９×１０３，病毒基因组由两条ＲＮＡ分子组成，大小分别为６．２ｋｂ与３．８ｋｂ，Ｎｏｒｔｈｅｒｎｂｌｏｔ结果显示３．８ｋｂＲＮＡ与ＢＢＷＶ２蚕豆分离物ＲＮＡ２ｃＤＮＡ有明显杂交反应．病毒粒子电泳后产生相似于豇豆花叶病毒科的快、慢两个电泳型．琼脂双扩散试验中，Ｓ１和ＢＢＷＶ２均能与蚕豆萎蔫病毒２（ＢＢＷＶ２）抗血清形成明显沉淀线，且沉淀线互相融合，Ｗｅｓｔｅｒｎ检测亦表明，此分离物的大小亚基均可与ＢＢＷＶ２抗血清反应．根据以上特性，Ｓ１分离物被鉴定为ＢＢＷＶ２．
A virus isolate S1 was obtained from the diseased soybean in the suburbs of Hangzhou. Fifteen plants belonging to 7 families were inoculated by artificial rubbing in the greenhouse, and S1 could infect 14 families of 5 families. S1 can be poisoned by Myzus persicae and Aphis gossypii in a non-persistent manner. The passivation temperature of this isolate is 50-55 ℃, the dilution limit is 10-3-10-4 and the protection period is 3-4 days 25 ° C). Hosts of quinoa breeding host may mention a large number of globular virions, d is 25-30 nm. The viral coat protein is mainly composed of two subunits, Mr respectively 44.7 × 103 and 21.9 × 103, the virus genome consists of two RNA molecules, the size was 6.2kb and 3.8kb, Northern blot results showed that 3 .8kbRNA and BBWV2 broad bean isolates RNA2cDNA obvious hybridization reaction. Virus particle electrophoresis produced similar to the cowpea mosaic virus fast, slow two electrophoresis. In agar double diffusion assay, both S1 and BBWV2 could form precipitated lines with the antiserum of broad bean wilt virus 2 (BBWV2), and the precipitates were fused with each other. Western analysis also showed that the size subunits of this isolate could be used with BBWV2 antiserum reaction. Based on the above characteristics, the S1 isolate was identified as BBWV2.