论文部分内容阅读
目的研究人死后骨骼肌肌钙蛋白I(Skeletal troponn in I,sTnI)的变化规律,探讨较晚期死后经过时间推断方法。方法以人胸大肌为研究对象,利用免疫印迹(W estern b lot)结合图象分析技术半定量检测不同离体时间内人胸大肌sTnI的含量,观察其与离体时间的关系。结果人胸大肌sTnI含量随离体时间延长而逐渐下降,与离体时间的对数值呈近似的线性关系:Y=11 972.5-4 761.9 lgX,相关系数r=0.989;离体5d的人胸大肌仍可检出sTnI。结论检验人死后骨骼肌(胸大肌)sTnI的含量有望成为用于推断人体较晚期死后经过时间的新技术。
Objective To study the variation of skeletal troponin I (sTnI) after death and to explore the method of estimating the later passage of time after death. Methods Human pectoralis major muscle was used as the research object. The content of sTnI in human pectoralis major muscle was detected semi-quantitatively by Western blot and image analysis technique. The relationship between sTnI and in vitro time was observed. Results The content of sTnI in human pectoralis major gradually decreased with prolongation of prolonged time and showed a linear relationship with the logarithm of ex vivo time: Y = 11 972.5-4 761.9 lgX, correlation coefficient r = 0.989; Large muscle still detectable sTnI. Conclusion It is expected that the content of sTnI in skeletal muscle (pectoralis major muscle) after death will become a new technique to infer the passage of time from the later death of the human body.