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目的对噪声暴露人群的miR-96与miR-183基因遗传变异性进行检测并探讨其意义。方法根据职业健康检查结果,从广州市某空调压缩机厂和某汽车发动机厂噪声作业人群中选取50名有职业性噪声性听力损伤(ONHL)的工人为病例组,以年龄、性别按1∶2配对选取的100名听力正常工人为对照组,采集血样,应用ABI3730XL测序仪检测该人群miR-96与miR-183基因的遗传变异,重点关注rs41274239、rs73159662、rs41281222、rs72631833的基因型,并与国际人群频率报道数据进行比较。结果 miR-96和miR-183基因的上述单核苷酸多态性突变频率均为0.000,与国际人群报道的频率基本一致;且在这2个基因中未发现新的突变。结论人群中miR-96和miR-183基因突变频率很低,开展相关研究对ONHL的预防和控制意义可能不大。
Objective To detect the genetic variability of miR-96 and miR-183 genes in noise-exposed population and explore its significance. Methods According to the result of occupational health examination, 50 workers with occupational noise-induced hearing impairment (ONHL) were selected from the air-conditioned compressor plant in Guangzhou City and the noise operation population of a car engine factory. The patients were divided into two groups according to the age and sex, 2 pairs of selected 100 normal hearing workers as the control group, blood samples were collected, the genetic variation of miR-96 and miR-183 genes was detected by ABI3730XL sequencer, and the genotypes of rs41274239, rs73159662, rs41281222 and rs72631833 were closely correlated with International crowd frequency reporting data for comparison. Results The mutation frequencies of miR-96 and miR-183 were all 0.000, which were consistent with those reported in the international population. No mutation was found in these two genes. Conclusions The frequencies of miR-96 and miR-183 gene mutations in the population are very low. It may not be meaningful to carry out relevant studies on the prevention and control of ONHL.