论文部分内容阅读
目的：探讨大黄对烫伤和内毒素二次打击后肝细胞内肿瘤坏死因子受体ＴＮＦＲ１ （ｐ５５）和ＴＮＦＲ２（ｐ７５）基因表达的影响。方法：应用随机、前瞻性研究方法，采用大鼠烫伤和内毒素二次打击模型，检测肝细胞ＴＮＦＲ１ 和ＴＮＦＲ２ 的基因表达情况以及血浆和肝组织内肿瘤坏死因子 α（ＴＮＦ α）和白介素 ６（ＩＬ ６）浓度。结果：原位杂交显示烫伤和内毒素二次打击后肝细胞内ＴＮＦＲ大量表达，以ＴＮＦＲ１ （ ｐ５５）为主，ＴＮＦＲ２ 仅小量表达，大黄（５０ ｍ ｇ／ｋｇ）治疗后两种受体表达均明显减少。烫伤和内毒素二次打击后血浆和肝组织内ＴＮＦ α浓度均明显增高，尤以肝组织内为甚（Ｐ＜ ０．０１）；大黄治疗后ＴＮＦ α浓度明显降低（Ｐ＜ ０．０１）。大黄亦能显著降低血浆内ＩＬ ６浓度（Ｐ＜ ０．０１）。结论：大黄可抑制病理情况下ＴＮＦＲ１ 、ＴＮＦＲ２ 表达，降低血浆和肝组织内ＴＮＦ α和ＩＬ ６ 水平，具有明显抗炎作用。
Objective: To investigate the effect of rhubarb on the expression of TNFR1 (p55) and TNFR2 (p75) genes in hepatocytes after scald and after two shots of endotoxin. METHODS: Randomized, prospective studies were performed using rat scald and endotoxin-two-hit models to detect hepatocyte TNFR1 and TNFR2 gene expression and plasma and liver tissue tumor necrosis factor alpha (TNF alpha) and interleukin 6 ( IL 6) concentration. RESULTS: In situ hybridization showed a large amount of TNFR expression in hepatocytes after scald and secondary tossing of endotoxin, mainly TNFR1 (p55), TNFR2 was only expressed in small amounts, and both receptors were expressed after treatment with rhubarb (50 m g/kg). Obviously reduced. After burns and two shots of endotoxin, the concentrations of TNFα in plasma and liver tissue were significantly increased, especially in liver tissue (P<0.01); TNFα concentration was significantly decreased after rhubarb treatment (P<0.01). . Rhubarb also significantly reduced plasma IL 6 concentrations (P < 0.01). Conclusion: Rhubarb can inhibit the expression of TNFR1 and TNFR2 in pathological conditions, reduce the levels of TNFα and IL 6 in plasma and liver tissues, and have obvious anti-inflammatory effects.