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本研究建立以离子液体作为萃取溶液,聚丙烯中空纤维膜作为支载体,三相微萃取尿液中的目标物质,以期建立阿片类毒品的高效液相色谱检测方法(IL-HF-LPME-HPLC)。实验以吗啡、曲马多、可待因、美沙酮四种阿片类毒品为研究对象,优化出最佳的HF-LPME的萃取模式和条件为:萃取剂为3-辛基-3-甲基咪唑六氟磷酸离子液体加15%三辛基氧化膦;接收相均为p H为1,加入Na Cl为2 mol/L,搅拌速度为1 000 r/min,萃取时间为60 min。通过本优化的方法检测4种阿片类物质,获得了较高的富集倍数(98~178倍)和较低的检测限(10~50 ng/m L),相对标准偏差(RSD)均为小于10%。研究表明,中空纤维膜起到了很好的隔离复杂基质中的大分子干扰作用,具有十分良好的富集效果。
In this study, IL-HF-LPME-HPLC with ionic liquid as extraction solution, polypropylene hollow fiber membrane as support and three-phase microextraction of target substance in urine to establish opioid drug was established. ). Experiments with morphine, tramadol, codeine, methadone four opiates drugs for the study to optimize the extraction of the best HF-LPME mode and conditions: extraction agent for the 3-octyl-3-methyl imidazole Hexafluorophosphate ionic liquid plus 15% trioctylphosphine oxide; the receiving phase were p H was 1, Na 2 C was added 2 mol / L, the stirring speed was 1000 r / min, the extraction time was 60 min. Four opioids were detected by this optimized method, and the higher enrichment factor (98 ~ 178 times) and lower limit of detection (10 ~ 50 ng / m L) were obtained. The relative standard deviations (RSDs) Less than 10%. Studies have shown that hollow fiber membranes play a very good isolation of macromolecules in complex matrix interference, has a very good enrichment effect.