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研究ATP-MgCl_2对缺血后肝组织的作用。方法:家兔全肝缺血30分钟后,分别给予生理盐水、ATP和ATP-MgCl_2处理,术后第1天,第3天和第5天取血测定谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)和碱性磷酸酶(ALP),并分别检测各组肝组织学的改变。结果:术后第1天ATP-MgCl_2组ALT明显低于对照组(P<0.05),而ATP组与对照组相比无显著差别:ATP-MgCl_2及ATP组的LDH和ALP与对照组相比无显著差别。术后24小时超微结构示对照组肝细胞明显水肿、细胞器严重损伤和血窦内微小血栓;而ATP-MgCl_2组细胞轻度水肿,细胞器基本正常,血窦内无微小血栓。结论:ATP-MgCl_2对缺血肝组织确有保护作用,其机制可能是通过改善微循环和减轻细胞损伤而起作用。
To study the effect of ATP-MgCl 2 on ischemic liver tissue. Methods: The rabbits were subjected to normal saline, ATP and ATP-MgCl 2 treatment for 30 minutes after ischemia. Blood samples were taken for ALT, (LDH) and alkaline phosphatase (ALP). The changes of liver histology in each group were detected. Results: The ALT in ATP-MgCl 2 group was significantly lower than that in control group on the first day after operation (P <0.05), but there was no significant difference between ATP group and control group. LDH and ALP in ATP-MgCl 2 and ATP group were significantly lower than those in control group No significant difference. At 24 hours after operation, the ultrastructure of the control group showed marked edema of hepatocytes, severe organelles’ severe damage and tiny blood clots in the sinusoids. However, the cells in the ATP-MgCl 2 group had mild edema, organelles were basically normal, and no micro-thrombus was found in the sinusoids. Conclusion: ATP-MgCl 2 has a protective effect on ischemic liver tissue, and its mechanism may be through improving microcirculation and reducing cell damage.