论文部分内容阅读
一、中性粒细胞脱氧核糖核酸染色法:福氏反应法(一) 试剂: 1.西佛Schiff试剂;硷性复红1.0克逐小量加入到200毫升沸水中,溶解后冷却到60—50℃时过滤,于滤液加入20毫升1N盐酸溶液,冷却到25℃下加入亚硫酸钠2克,活性炭0.3克摇匀,塞紧瓶口,保存于暗冷处,24小时后才用,用前趁冷过滤,滤液应为无色。如溶液变红则失效。2.二氧化硫水溶液:10%亚硫酸钠溶液5毫升,加1N盐酸5毫升,加水至100毫升。临同前配。
First, neutrophil DNA staining: Freescale reaction method (a) Reagents: 1. West Buddha Schiff reagent; alkaline complex red 1.0 grams by small amount added to 200 ml of boiling water, dissolved and cooled to 60- 50 ° C filtered, 20 ml of 1N hydrochloric acid solution was added to the filtrate, cooled to 25 ° C by adding 2 g of sodium sulfite, activated carbon 0.3 g shaken, bottle tightly, stored in the dark place, 24 hours before use, before use Cold filtration, the filtrate should be colorless. If the solution becomes red then failure. 2. Sulfur dioxide solution: 5 ml of 10% sodium sulfite solution, 5 ml of 1N hydrochloric acid and water to 100 ml. Pro front match.