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我们根据Jamjoom的报道(J Clin Microbiol1983;17:717)用暗视野显微镜观察不染色血片的疟原虫(下称暗视野法),对52例间日疟病人和15名健康人作了检测。 材料和方法 一、血标本:取临床诊断的现症间日疟病人和当地健康人指尖血适量,每人涂6张薄血片,其中暗视野法用22×50mm的盖玻片涂2张,其余按常规用瑞氏染色和吖啶橙染色各2张。 二、暗视野法操作程序:(一)配制缓冲甘油液:仿Jamjoom法,甘油90ml、水10ml、碳酸钠0.16g、碳酸氢钠0.0715g。
We examined 52 malaria-naïve individuals and 15 healthy individuals using a dark-field microscope to observe non-stained blood samples of Plasmodium (hereinafter referred to as “dark field”) according to the report of Jamjoom (J Clin Microbiol 1983; 17: 717). Materials and methods First, the blood specimens: Clinically diagnosed patients were infected with Plasmodium falciparum and local healthy people finger tip blood volume, each coated with 6 thin blood films, including dark field with 22 × 50mm cover glass 2 Zhang, the rest by conventional Wright staining and acridine orange staining of 2. Second, dark field method of operation procedures: (a) preparation of buffer glycerin solution: imitation Jamjoom law, glycerol 90ml, water 10ml, sodium carbonate 0.16g, sodium bicarbonate 0.0715g.