目的研究椒蒿挥发油对小鼠自身免疫性睾丸炎睾丸组织结构及血清抑制素B和卵泡刺激素含量的影响。方法健康成年雄性BALB/c小鼠150只,随机分为生理盐水组、模型组及椒蒿低、中、高剂量组(即椒蒿挥发油的体积分数为0.938%、1.875%、3.375%),采用单侧睾丸注射冰醋酸的方法造模。各组都在造模前3d开始灌胃,手术并单侧睾丸注射冰醋酸后继续灌胃,连续灌胃24d后,分别于术后3周、6周、9周取材检测,光镜观察非手术侧睾丸生精小管内的变化,ELISA法检测血清抑制素B和卵泡刺激素的变化情况。结果术后3、6、9周,模型组及椒蒿低、中剂量组血清抑制素B含量均低于生理盐水组,差异有统计学意义(P<0.05),卵泡刺激素含量均高于生理盐水组,差异有统计学意义(P<0.05),术后9周,椒蒿高剂量组血清抑制素B和卵泡刺激素含量与生理盐水组比较,差异无统计学意义(P>0.05),术后9周,椒蒿高剂量组光镜下睾丸组织结构基本正常。结论椒蒿挥发油对小鼠自身免疫性睾丸炎的发生有一定的拮抗作用,同时也跟剂量有关,高剂量组的拮抗效果最好,术后灌胃9周,睾丸组织结构基本恢复正常。 Objective To study the effect of volatile oil of Artemisia annua on testis tissue structure, serum inhibin B and follicle stimulating hormone in mice with autoimmune orchitis. Methods 150 healthy adult male BALB / c mice were randomly divided into saline group, model group and low, medium and high doses of Artemisia ordosica (ie, the volume fraction of essential oil of Artemisia annua was 0.938%, 1.875%, 3.375%), Unilateral testicular injection of acetic acid method of modeling. The rats in each group were given gavage three days before the model making. After the operation and unilateral testicular injection of glacial acetic acid, gavage was continued. After continuous gavage for 24 days, samples were taken at 3 weeks, 6 weeks and 9 weeks after operation respectively. Surgical side of the testicular seminiferous tubule changes, ELISA assay serum statin B and follicle-stimulating hormone changes. Results The levels of serum inhibin B in model group and low and medium doses of Artemisia annua were lower than those in normal saline group at 3, 6 and 9 weeks after operation (P <0.05), and the levels of follicle-stimulating hormone (P <0.05). At 9 weeks after operation, the content of serum inhibin B and follicle stimulating hormone in the high dose group of Artemisia ordosica had no statistical difference (P> 0.05) At 9 weeks after operation, the tissue structure of testis in high dose group was basically normal. Conclusions The volatile oil of Artemisia annua antagonizes the occurrence of autoimmune orchitis in mice and is also related to the dose. The antagonistic effect of the high dose group is the best. After the operation for 9 weeks, the testicular tissue structure returned to normal.