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[目的]建立一种高效液相色谱法测定三唑酮·丙环唑缓释微球的定量分析方法。[方法]采用高效液相色谱法,以甲醇和水(体积比85∶15)为流动相,流速为1 m L/min,以230 nm为检测波长,对试样中三唑酮和丙环唑进行液相色谱分离测定。[结果]三唑酮和丙环唑的保留时间分别为4.293、5.673 min,线性相关系数分别为0.9971、0.9983,标准偏差分别为0.1031、0.0946,变异系数分别为1.0757%、0.6634%;平均回收率分别为99.01%、99.23%。[结论]该方法操作简便,分离效果好,精密度与准确度高,可同时测定三唑酮和丙环唑含量。
[Objective] To establish a method for the quantitative analysis of triadimefon propiconazole sustained-release microspheres by high performance liquid chromatography. [Method] The mobile phase of methanol and water (volume ratio 85:15) was used as mobile phase at a flow rate of 1 m L / min and the detection wavelength was 230 nm. The contents of triadimefon and propane ring Azole was subjected to liquid chromatography. [Results] The retention times of triadimefon and propiconazole were 4.293 and 5.673 min, respectively. The linear correlation coefficients were 0.9971 and 0.9983. The standard deviations were 0.1031 and 0.0946, respectively. The coefficients of variation were 1.0757% and 0.6634%, respectively. The average recoveries Respectively, 99.01%, 99.23%. [Conclusion] The method was simple and easy to operate with good separation effect and high precision and accuracy. The method could simultaneously determine the contents of triadimefon and propiconazole.