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目的:利用生物活性-液质联用跟踪方法快速分离、鉴定苦参中的酪氨酸酶抑制成分。方法:利用生物活性-液质联用跟踪方法,通过接收器接样,并用酪氨酸酶抑制作用强度,快速查找苦参中的酪氨酸酶抑制成分。结果:苦参的甲醇提取物及其正丁醇萃取物显示较强的酪氨酸酶抑制作用。从苦参的正丁醇萃取物中分离并鉴定2个黄酮类化合物,即槐属二氢黄酮G和苦参酮。与已知的酪氨酸酶抑制剂曲酸(IC50=90.08μmol.L-1)相比,槐属二氢黄酮G和苦参酮具有更强的酪氨酸酶抑制作用,其半数抑制率(IC50)值分别为7.03和5.06μmol·L-1。结论:生物活性-液质联用跟踪方法是一种可以简单、快速确定中药活性成分的方法。
OBJECTIVE: To rapidly isolate and identify tyrosinase inhibitory elements in Sophora flavescens by bioactivity-LC-MS method. Methods: The bioactivity - LC / MS method was used to detect the tyrosinase inhibitory component in Sophora flavescens. Results: The methanol extracts of Sophora flavescens and its n-butanol extract showed strong tyrosinase inhibition. Isolation and identification of two flavonoids from the n-butanol extracts of Sophora flavescens, namely, S. huperzepohn and matrine. Compared with the known tyrosinase inhibitor kojic acid (IC50 = 90.08μmol.L-1), the genotypes of Sophora flavescens G and matrine had stronger tyrosinase inhibition, and the half-inhibitory rate (IC50) were 7.03 and 5.06μmol·L-1, respectively. Conclusion: Bioactive-LC-MS method is a simple and rapid method to determine the active ingredients of traditional Chinese medicine.