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目的:通过强光照射抑制鸡褪黑素的分泌,诱导与人特发性脊柱侧凸相类似的脊柱侧凸畸形,以探讨褪黑素与特发性脊柱侧凸的关系。方法:30只1d龄雏鸡,置于24h持续强光照射的环境中,另外10只相同的小鸡置于普通光照环境中,光照时间为7Am至7Pm。从第2周开始,每周给小鸡摄脊柱片观察,另外在第4周2Am及2Pm时取静脉血用ELISA方法检测血清褪黑素水平,第6周时将小鸡处死。结果:①实验组实验结束时存活的25只中有7只出现了脊柱侧凸,发生率为(7/25)28%,Cobb角10°~14°;对照组鸡均未出现脊柱畸形。②4周时对照组鸡2Pm和2Am时血清褪黑素水平分别为51.02±3.74pg/ml和193.84±12.25pg/ml,表现出明显的昼低夜高的节律性分泌特征。③实验组鸡无论脊柱侧凸发生与否,2Am和2Pm时血清褪黑素水平分别为49.98±2.42pg/ml和50.60±3.15pg/ml,其节律性分泌特征消失。结论:昼夜持续强光照射可以抑制鸡褪黑素的分泌,使其丧失昼夜节律性分泌,并可诱导与人特发性脊柱侧凸相似的鸡脊柱侧凸畸形。
OBJECTIVE: To inhibit the secretion of melatonin by intense light irradiation and to induce scoliosis deformity similar to human idiopathic scoliosis to explore the relationship between melatonin and idiopathic scoliosis. Methods: Thirty 30-day-old chicks were exposed to continuous light for 24 hours and the other 10 identical chicks were placed in normal light with a light exposure of 7-7 mpm. From the second week onwards, the chickens were observed for vertebral column weekly, and the venous blood was taken at 2Am and 2Pm of the fourth week to detect the level of serum melatonin. At the sixth week, the chickens were sacrificed. Results: ① In the experimental group, 7 out of 25 surviving ones showed scoliosis, with a rate of (7/25) 28% and a Cobb angle of 10 ° ~14 °. No spinal deformity occurred in the control group. ② The serum melatonin levels of 2Pm and 2Am in control group at 4 weeks were 51.02 ± 3.74pg / ml and 193.84 ± 12.25pg / ml, respectively, showing obvious rhythmic secretion characteristics at daytime and nighttime. ③In the experimental group, whether the scoliosis occurred or not, the level of serum melatonin at 2Am and 2Pm was 49.98 ± 2.42pg / ml and 50.60 ± 3.15pg / ml respectively, and the rhythmic secretion disappeared. CONCLUSION: Continuous light and strong radiation day and night can inhibit the secretion of melatonin from chickens, causing it to lose circadian rhythmic secretion and inducing chick scoliosis deformity similar to human idiopathic scoliosis.