论文部分内容阅读
用犬弓蛔虫(Toxocara canis)Ⅱ期幼虫对160种斯里兰卡传统药的热水提取物的杀线虫活性进行了筛选试验,方法如下: 每种生药15g切碎后用热水(150ml×2)提取,每次2h。提取液用棉花过滤后减压浓缩,冷冻干燥。将冻干粉末溶于0.75％盐水配成10mg/ml的药液。若药液发浑则用Tokyo Rosi 2号滤纸过滤,备用。取20条幼虫置于Corning池中,加上述药液在37℃时孵化。孵化开始后1,3,6和24h在显微镜下观察幼虫行为。每个试
The nematicidal activity of hot water extracts from 160 traditional Sri Lankan medicines was tested using a second-instar larva of Toxocara canis as follows: 15 g of each crude drug was chopped and extracted with hot water (150 ml × 2) , Each 2h. The extract was filtered through cotton, concentrated under reduced pressure and freeze-dried. The lyophilized powder was dissolved in 0.75% saline dubbed 10mg / ml of liquid. If the solution is cloudy, filter it with Tokyo Rosi No. 2 filter and set aside. Twenty larvae were placed in Corning ponds and incubated at 37 ° C. The behavior of larvae was observed microscopically at 1, 3, 6 and 24 h after hatching. Each test