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观察了枯草杆菌α-淀粉酶、蛋白酶在聚乙二醇/硫酸铵和聚乙二醇/磷酸盐两个双水相系统中的分配行为。设计了借液-液萃取方式分别部分纯化上述两酶粗品的方案。经部分纯化后α-淀粉酶的比活力(按每毫克制品含活力单位数计算)提高至6倍,活力回收率可达80%;蛋白酶比活力提高至8倍,活力回收率在50～60%间。此法具有简易、快速、有效、并可准确放大缩小操作规模等特点。
The distribution of Bacillus subtilis α-amylase and protease in polyethylene glycol / ammonium sulfate and polyethylene glycol / phosphate aqueous two-phase system was observed. The scheme of partially purifying the above two crude enzymes by liquid-liquid extraction was designed. After partial purification, the specific activity of α-amylase (calculated by the unit of activity per milligram of product) increased to 6 times, the recovery rate of vitality up to 80%; the specific activity of protease increased to 8 times and the recovery of viability from 50 to 60 %between. This method is simple, fast, effective, and can be accurately scaled to reduce the size and other characteristics.