利用垂体细胞移植治疗某些垂体功能不足的疾病,在国内外业已开展。为增加供体来源,在探索垂体保存的过程中,我们对5例鼠垂体细胞在4℃条件下保存的结果进行了观察,现报告如下。 1.将小鼠断颈处死,以碘酒、酒精常规消毒鼠的头颈部。打开鼠颅骨暴露垂体,用眼科剪、镊取出垂体置生理盐水中漂洗一次后,再用RPMI-1640保养液漂洗一次。在超净台中将漂洗过的鼠垂体置2ml 1640液中(含青霉素40u/ml、链霉素40u/ The use of pituitary cell transplantation in the treatment of some pituitary insufficiency diseases have been carried out at home and abroad. In order to increase the donor source, during the exploration of the preservation of the pituitary, we observed the results of preservation of 5 cases of pituitary cells at 4 ° C and are reported as follows. 1. The mice were sacrificed by cervical dislocation to iodine, alcohol, conventional disinfection of rats head and neck. Open the rat skull exposed pituitary, with ophthalmic scissors, tweezers removed pituitary placed in saline once again rinsed once again with RPMI-1640 maintenance solution. Taichung will rinse off the pituitary gland placed 2ml 1640 solution (containing penicillin 40u / ml, streptomycin 40u /