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目的通过研究中药复方Formula-3(乌梅、灵芝、人参、当归、黄连、干姜)对嗜碱性粒细胞系(RBL-2H3细胞)和SOC通道的作用,探讨Formula-3对肥大细胞脱颗粒的作用机制。方法体外培养大鼠RBL-2H3细胞系制作肥大细胞的模型,通过MTT实验、脱颗粒实验和激光共聚焦(Confocal)实验来研究Formula-3对肥大细胞脱颗粒的作用机制。结果 Formula-3(终质量浓度0.05、0.2、0.8 mg/ml)在急性(0.5 h)和慢性(12 h)作用下呈质量浓度依赖性的抑制致敏RBL-2H3细胞脱颗粒(P<0.01),而对非致敏RBL-2H3细胞没有影响(P>0.05)。Formula-3可显著的抑制SOC通道钙离子内流(P<0.01)。结论 Formula-3通过抑制SOC通道钙离子内流来显著抑制肥大细胞脱颗粒,为治疗过敏性疾病提供理论依据。
Objective To study the effect of Formula-3 (Fructus Mume, Ganoderma lucidum, Ginseng, Angelica, Coptis, Ginger) on basophil cell line (RBL-2H3) The mechanism of action. Methods The mast cell model was established by culturing rat RBL-2H3 cells in vitro. The mechanism of action of Formula-3 on mast cell degranulation was studied by MTT assay, degranulation test and confocal test. Results Formula-3 (0.05, 0.2, 0.8 mg / ml) inhibited the sensitization of RBL-2H3 cells degranulation in a concentration-dependent manner (P <0.01 ), But had no effect on non-sensitized RBL-2H3 cells (P> 0.05). Formula-3 significantly inhibited the SOC channel calcium influx (P <0.01). Conclusions Formula-3 can inhibit mast cell degranulation by inhibiting the influx of calcium in SOC channel and provide a theoretical basis for the treatment of allergic diseases.