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切取嘉兰（ＧｌｏｒｉｏｓａｒｏｔｈｓｃｈｉｌｄｉａｎａＯ’Ｂｒｉｅｎ）０．５ｍｍ左右的茎尖组织接种在Ｍｕｒａｓｈｉｇｅ＆Ｓｋｏｏｇ培养基上，在２５℃、１６ｈ光照（１０００ｌｘ）条件下培养１个月可以获得再生植株。继代培养后，这些再生植株可以在试管内形成块茎。再生植株的茎基部分生能力较强，利用其茎盘反复继代培养可以获得大量不定芽，再经过分株培养可以形成块茎，从而实现大量繁殖的目的。当培养基内的６－ＢＡ浓度提高到４ｍＬ／Ｌ时，可获得最多的不定芽和较大的茎盘。提高蔗糖浓度可以获得较大的块茎，当蔗糖浓度提高到９％时，块茎平均单重达０．４８ｇ。
The cuttings of about 0.5 mm shoots of Gloriosarothschildiana O’Brien were inoculated on Murashige & Skoog medium and regenerated at 25 ° C for 16 months at 1000 lx for 1 month. After subculture, these regenerated plants can form tubers in vitro. Regeneration plant stem base strong ability to abortion, the use of its stems repeatedly subculture can get a large number of adventitious buds, and then through ramet culture can form tubers, so as to achieve the purpose of mass reproduction. When the 6-BA concentration in the medium was increased to 4 mL / L, the largest number of adventitious buds and larger stem discs were obtained. Larger tubers can be obtained by increasing the sucrose concentration, with an average single weight of 0.48 g when the sucrose concentration was increased to 9%.