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目的研究凋亡相关基因程序化死亡因子5(PDCD5)在胃癌组织的表达情况。方法应用免疫组织化学方法 ,使用鼠抗人PDCD5单克隆抗体,检测60例胃癌组织、60例癌旁组织及20例正常胃黏膜中的PDCD蛋白的表达情况。结果胃癌组织中PDCD5蛋白表达率为35.0%、癌旁组织中PDCD5阳性表达率为63.3%、正常胃组织PDCD5阳性率为85.0%。胃癌组织中PDCD5表达率分别为高分化组53.8%、中分化组47.3%、低/未分化组17.2%。PDCD5在胃癌组织中低表达或不表达,与胃癌患者的性别、年龄、肿瘤的浸润深度以及临床分期无相关性。与胃癌的分化程度及是否伴有淋巴结转移相关。结论 PDCD5蛋白在胃癌组织中表达率低于正常组织,PDCD5在胃癌细胞和胃肿瘤细胞凋亡过程中起到关键作用。
Objective To study the expression of apoptosis-related gene programmed death factor 5 (PDCD5) in gastric cancer. Methods Immunohistochemical method was used to detect the expression of PDCD protein in 60 cases of gastric cancer, 60 cases of paracancerous tissues and 20 cases of normal gastric mucosa by using mouse anti-human PDCD5 monoclonal antibody. Results The positive rate of PDCD5 in gastric cancer tissue was 35.0%, the positive rate of PDCD5 in adjacent tissues was 63.3%, and the positive rate of PDCD5 in normal gastric tissues was 85.0%. The positive rates of PDCD5 in gastric cancer tissues were 53.8% in well-differentiated group, 47.3% in moderately differentiated group and 17.2% in poorly differentiated group. The expression of PDCD5 in gastric cancer was not correlated with the gender, age, depth of tumor invasion and clinical stage. And gastric cancer differentiation and whether associated with lymph node metastasis. Conclusion The expression of PDCD5 protein in gastric cancer tissues is lower than that in normal tissues. PDCD5 plays a key role in the apoptosis of gastric cancer cells and gastric cancer cells.