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为简化杂交小麦制种环节,扩大和创新小麦温敏雄性不育遗传资源,在提莫菲维小麦细胞质背景下利用莫迦小麦1B染色体上的T型恢复基因作为选择标记,将与其连锁的莫迦小麦雄性不育温敏基因导入普通小麦3314的核基因组,获得带有莫迦小麦1B染色体有关育性片断的材料TM3314,经回交导入粘果山羊草细胞质背景,育成KTM3314A(简称为YM3314)。经秋播、秋播剪穗再生分蘖、夏播、春播试验,YM3314在陕西杨陵秋播雄性不育,秋播剪穗再生分蘖、夏播、春播均雄性可育;结合人工气候箱控温控光试验结果表明,YM3314的雄性不育具有温度敏感特性,对日长似乎不敏感。带有莫迦小麦温敏雄性不育基因的小麦雄性不育系,可称作YM型小麦温敏不育系。
In order to simplify the process of hybrid wheat seed production and to expand and innovate the genetic resources of wheat thermo-sensitive male sterile, the T-type restorer gene on wheat 1B chromosome of Mozambique was selected as the selection marker in Timofeev wheat cytoplasm background, Male sterility thermo-sensitive gene was introduced into the nuclear genome of common wheat 3314 to obtain TM3314 with the fertility-related fragment of wheat 1B chromosome. The recombinant plasmid was introduced back into the cytoplasm of Aegilops tauschii and back to KTM3314A (abbreviated as YM3314). After the autumn sowing, autumn sowing spike regeneration tillering, summer sowing and spring sowing test, YM3314 was male sterile in Yangling autumn in Shaanxi Province, tillering in autumn sowing spike, and male fertile in summer sowing and spring sowing. Combined with artificial climate control The results of the light test showed that male sterility of YM3314 was temperature-sensitive and appeared to be insensitive to Japanese growth. The wheat male sterile line with the Mowa wheat thermo-sensitive male sterile gene may be referred to as the YM wheat thermo-sensitive male sterile line.