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脱水素(dehydrin,DHN)属于LEA蛋白第二家族成员,是一种植物中广泛存在的亲水性蛋白,在干旱、低温和高盐等非生物胁迫的环境中起到重要作用。通过生物信息学方法对玉米全基因组DHN家族成员进行了鉴定,并进一步对其系统发育、基因结构、染色体定位和基因复制以及表达模式进行了系统分析。结果显示,在玉米DHN基因家族中共有5个家族成员,多物种系统发育进化树分析、基因结构以及基序分析都表明DHN家族成员在进化上具有高度的保守性。基因复制和物种间微共线性分析表明,在5个玉米DHN基因中存在着1对片段复制基因(ZmDHN1-ZmDHN2),玉米、高粱和水稻3个物种间存在2对直系同源基因(ZmDHN2-Sb04g032250.1,ZmDHN2-Os02g44870.1)。通过转录组表达数据分析表明,玉米DHN家族基因在不同发育时期具有不同的组织表达模式;同时,诱导表达模式分析表明ZmDHN基因的表达受到盐和干旱胁迫的显著诱导。该研究结果将为进一步鉴定玉米DHN家族重要的基因成员并对其开展功能分析奠定基础。
Dehydrin (DHN), a member of the second family of LEA proteins, is a hydrophilic protein widely found in plants and plays an important role in abiotic stress conditions such as drought, cold and salt stress. The genome-wide DHN family members of maize were identified by bioinformatics methods, and their phylogeny, gene structure, chromosomal location, gene duplication and expression patterns were systematically analyzed. The results showed that there are 5 family members in the DHN gene family of maize, phylogenetic tree analysis of multiple species, gene structure and motif analysis indicate that DHN family members are highly conserved in evolution. Gene duplication and inter-species collinearity analysis showed that there were 1 pair of fragment replicating genes (ZmDHN1-ZmDHN2) in 5 maize DHN genes and 2 pairs of orthologous genes in maize, sorghum and rice (ZmDHN2- Sb04g032250.1, ZmDHN2-Os02g44870.1). The analysis of transcriptome expression data showed that the DHN family of maize had different tissue expression patterns at different developmental stages. Meanwhile, the expression pattern of ZymDHN gene was significantly induced by salt and drought stress. The results of this study will lay the foundation for further identification and functional analysis of important DHN family members.