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探讨了马蹄金 (DichondrarepensForst.)下胚轴离体培养的方法。在黑暗条件下种子萌发培养无菌苗 ,可迅速获得大量下胚轴 ,然后将下胚轴接种到MS + 0 .2mg L 2 ,4-D + 0 .5mg L 6-BA中可迅速获得大量愈伤组织 ,再用B5 + 2mg L 6-BA + 0 .2mg Lα -NAA作分化培养基出苗效果较好。
The method of in vitro culture of hypocotyls from Dichondra repens Forst. Seed germination under dark conditions sterile seedlings can be quickly obtained a large number of hypocotyls, and then inoculated hypocotyls MS +0. 2mg L 2, 4-D +0. 5mg L 6-BA can quickly obtain a large number of Callus, and then B5 +2 mg L 6-BA +0 .2mg Lα-NAA for differentiation medium emergence effect is better.