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目的 观察芪归合剂对肾病综合征鼠 (NS鼠 )胰岛素样生长因子 Ⅰ (IGF Ⅰ )、胰岛素样生长因子结合蛋白 3 (IGFBP 3 )的作用。方法 制备大鼠阿霉素NS模型 ,设正常对照组、NS组、NS蒸馏水治疗组和NS芪归合剂治疗组 ,每组 5只大鼠。以放射免疫法和免疫放射法测定各组血、尿IGF I和IGFBP 3水平 ;用RT PCR法检测肝IGF ⅠmRNA、IGFBP 3mRNA表达 ;测量鼠的身长、体重和血生化指标。结果 NS组鼠体重增长 [( 4 7± 3 4 )g/ 4周 ]、身长增长 [( 2 6± 0 8)cm/ 4周 ]、血IGF Ⅰ [( 4 91±66) μg/L]、IGFBP 3 [( 10 2± 4 ) μg/L]低于正常鼠组 [分别为 ( 162± 19)g/ 4周、( 8 1± 1 5 )cm/ 4周、( 968±184 ) μg/L、( 13 3± 16) μg/L],尿IGF Ⅰ [( 2 64± 119)ng/ 2 4h]高于正常鼠组 [( 5 9± 16)ng/ 2 4h],而肝IGF ⅠmRNA表达 ( 0 5 2± 0 0 4 )与正常对照组 ( 0 5 3± 0 0 6)相似、IGFBP 3mRNA表达 ( 0 5 6± 0 0 5 )低于正常鼠组 ( 0 93± 0 0 5 )。芪归合剂治疗组肝IGF ⅠmRNA表达 ( 0 93± 0 0 5 )、IGFBP 3mRNA表达 ( 0 87± 0 0 5 )高于NS蒸馏水治疗组。结论 NS鼠存在IGF Ⅰ、IGFBP 3代谢紊乱和生长障碍 ,血IGF Ⅰ降低主要是由于尿中排出所致 ,而血IGFBP 3降低主要与合成减少有关。
Objective To observe the effects of guangui mixture on insulin-like growth factor I (IGF I) and insulin-like growth factor binding protein 3 (IGFBP 3) in rats with nephrotic syndrome (NS). Methods The rat adriamycin NS model was prepared. The normal control group, the NS group, the NS distilled water treatment group, and the NS Qigui mixture treatment group were used. Each group had 5 rats. The levels of IGF I and IGFBP 3 in blood and urine were measured by radioimmunoassay and immunoradiometric assay. The mRNA expression of IGF I mRNA and IGFBP 3 in liver were detected by RT PCR. The length, body weight and blood biochemical parameters of mice were measured. Results Body weight gain in NS group [(47±34) g/4 weeks], length increase [(26±08) cm/4 weeks], blood IGF I [(4 91±66) μg/L] IGFBP 3 [( 10 2 ± 4) μg/L] was lower than that in the normal group [(162 ± 19) g / 4 weeks, (8 1 ± 1 5) cm / 4 weeks, (968 ± 184) μg] /L, (13 3 ± 16) μg/L], urinary IGF I [(2 64± 119) ng/24h] is higher than that of normal mice [(5 9±16) ng/24h], and liver IGF The mRNA expression of I mRNA was similar to that of the normal control group ( 0 5 3±0 0 6) and the expression of IGFBP 3 mRNA (0 5 6± 0 0 5) was lower than that of the normal group ( 0 93 ± 0 0 5). ). In the treatment group, the expression of IGF I mRNA (0 93±05) and IGFBP 3 mRNA (0 87± 0 05) were higher in the treatment group than in the NS distilled water treatment group. Conclusion There are IGF I and IGFBP 3 metabolic disorders and growth disorders in NS mice. The decrease of blood IGF I is mainly due to urinary excretion, and the decrease of blood IGFBP 3 is mainly related to the decrease of synthesis.