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近年来发展的酵母人工染色体YeastArtificalChromosome(YAC)可以克隆长至几百kb的DNA片段。目前世界上已有一些YAC文库,可以用PCR方法或传统的原位杂交法来筛选高密度克隆膜以得到单一YAC克隆,建立染色体特定区域的YAC重...
The Yeast Artifical Chromosome (YAC), a yeast artificial chromosome developed in recent years, can clone DNA fragments up to several hundred kb in length. At present, there are some YAC libraries in the world that can screen high-density clone membrane by PCR method or traditional in situ hybridization method to get a single YAC clone and establish YAC heavy ...