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研究三苯氧胺(Tamoxifen,TAM)对人脑胶质瘤细胞辐射增敏作用及诱导细胞凋亡。用3H-TdR掺入法、免疫组织化学法、流式细胞术和激光共聚焦显微镜扫描技术检测细胞DNA合成、雌激素受体表达、细胞凋亡及凋亡的形态学特征。结果表明,TAM能抑制细胞DNA合成,与60Coγ线联用抑制作用增强,表现出协同作用。TAM能诱导细胞凋亡,其凋亡率随TAM浓度的升高而增高,凋亡细胞核固缩和核碎裂,可见凋亡小体。而免疫组化结果显示SHG-44细胞不表达雌激素受体。以上结果表明TAM可能通过诱导细胞凋亡途径来增加细胞的辐射敏感性,而与雌激素受体途径无关。
To study the effect of Tamoxifen (TAM) on the proliferation of human glioma cells and the induction of apoptosis. 3H-TdR incorporation, immunohistochemistry, flow cytometry and laser scanning confocal microscopy were used to detect the morphological characteristics of DNA synthesis, estrogen receptor expression, apoptosis and apoptosis. The results showed that TAM could inhibit the DNA synthesis and enhance the synergistic effect with 60Coγ-ray. TAM can induce apoptosis. The apoptosis rate of TAM increased with the increase of TAM concentration. Apoptotic nuclear pyknosis and nuclear fragmentation were observed, apoptotic bodies were observed. Immunohistochemistry showed that SHG-44 cells did not express estrogen receptor. The above results indicate that TAM may increase the radiation sensitivity of cells by inducing the apoptosis pathway, but not with the estrogen receptor pathway.