以高粱属约翰逊草和同源四倍体高粱四沈甜为指示材料,利用99对SSR引物比较了多态性检测中聚丙烯酰胺凝胶的两种染色方法,结果表明:由同一原理衍生出的两种染色方法有一定的差异,其中“强碱法”具有较强的适应性和稳定性,而且具有更高的效率,尤其在分子标记辅助选择中更适合。 Two kinds of dyeing methods of polyacrylamide gels in polymorphism detection were compared using 99 pairs of SSR primers with Sorghum johnsunatum and autotetraploid as sorghum tetraspin. The results showed that the two methods were derived from the same principle Of the two staining methods have some differences, including “strong alkali method” has strong adaptability and stability, but also has higher efficiency, especially in molecular marker-assisted selection more suitable.